Harvard University - Research Assistant Opportunity in Molecular and Experimental Evolution

A laboratory technician (research assistant) position is available with Dr. Betul Kacar within the Edwards Lab of Department of Organismic and Evolutionary Biology at Harvard University.

The researcher will contribute to the development of a new experimental approach that

involves reconstructing ancient molecular components and observing their biological function

in modern bacterial cells through experimental evolution. This will generate experimental

systems consisting of viable modern-ancient hybrid bacterial organisms amenable to

biological, biochemical and genetic characterization. The position will be based at the main

campus of Harvard University, and with access to resources available through the Organismic

and Evolutionary Department and the FAS Center for Systems Biology. The position includes

competitive salary and full benefits, and is for 1 year with potential extension to 2 or more



The minimum academic requirement for this posting is a master’s degree in molecular and cell

biology, genetics, biology, or a closely related field. Preference will be given to applicants with

at least 2 years of research experience or an equivalent combination of education and

experience that required the use of skills listed below.

Job Description:

Primary tasks: (80%)

• Preparation, routine care and aseptic handling of bacterial cultures. Preparation of

sterile reagents, chemical solutions, and bacteriological media. Preparation and

maintenance of bacterial freezer stocks, and preparation of microbial populations for

laboratory evolution and whole-genome sequencing analysis.

• Performance of laboratory molecular biological techniques, including but not limited to

DNA/RNA isolation, PCR, molecular cloning, microbial genome editing using

homologous recombination or other relevant methods, and basic DNA sequence


• Conducting pairwise bacterial fitness assays and viable colony counts.

Secondary tasks: (20%)

• Ordering lab supplies, setting up new lab equipment and procedures, laboratory

equipment testing, coordination of lab supply and culture sharing with collaborating

labs, training of new undergraduate students and postdocs.


• Ability to work odd hours if and as the project demands

• Attendance of relevant conferences to present research findings. Limited to 1 per

calendar year

• Regular presentation of research findings at lab meetings

• Maintenance of orderly, up-to-date written and electronic lab notebooks consistent with

standard NIH guidelines.

• Programming skills (e.g., R language) a plus.

This position is available immediately and will remain open until the position is filled.

For questions about the posting or the application process please contact Betul Kacar,

kacar@g.harvard.edu directly.

For more information about currently available opportunities, please visit http://kacarlab.org/join/

For further information about OEB please visit http://www.oeb.harvard.edu.

Harvard University is an equal opportunity employer and all qualified applicants will

receive consideration for employment without regard to race, color, religion, sex, sexual

orientation, gender identity, national origin, disability status, protected veteran status, or any

other characteristic protected by law.

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