A laboratory technician (research assistant) position is available with Dr. Betul Kacar within the Edwards Lab of Department of Organismic and Evolutionary Biology at Harvard University.
The researcher will contribute to the development of a new experimental approach that
involves reconstructing ancient molecular components and observing their biological function
in modern bacterial cells through experimental evolution. This will generate experimental
systems consisting of viable modern-ancient hybrid bacterial organisms amenable to
biological, biochemical and genetic characterization. The position will be based at the main
campus of Harvard University, and with access to resources available through the Organismic
and Evolutionary Department and the FAS Center for Systems Biology. The position includes
competitive salary and full benefits, and is for 1 year with potential extension to 2 or more
The minimum academic requirement for this posting is a master’s degree in molecular and cell
biology, genetics, biology, or a closely related field. Preference will be given to applicants with
at least 2 years of research experience or an equivalent combination of education and
experience that required the use of skills listed below.
Primary tasks: (80%)
• Preparation, routine care and aseptic handling of bacterial cultures. Preparation of
sterile reagents, chemical solutions, and bacteriological media. Preparation and
maintenance of bacterial freezer stocks, and preparation of microbial populations for
laboratory evolution and whole-genome sequencing analysis.
• Performance of laboratory molecular biological techniques, including but not limited to
DNA/RNA isolation, PCR, molecular cloning, microbial genome editing using
homologous recombination or other relevant methods, and basic DNA sequence
• Conducting pairwise bacterial fitness assays and viable colony counts.
Secondary tasks: (20%)
• Ordering lab supplies, setting up new lab equipment and procedures, laboratory
equipment testing, coordination of lab supply and culture sharing with collaborating
labs, training of new undergraduate students and postdocs.
• Ability to work odd hours if and as the project demands
• Attendance of relevant conferences to present research findings. Limited to 1 per
• Regular presentation of research findings at lab meetings
• Maintenance of orderly, up-to-date written and electronic lab notebooks consistent with
standard NIH guidelines.
• Programming skills (e.g., R language) a plus.
This position is available immediately and will remain open until the position is filled.
For questions about the posting or the application process please contact Betul Kacar,
For more information about currently available opportunities, please visit http://kacarlab.org/join/.
For further information about OEB please visit http://www.oeb.harvard.edu.
Harvard University is an equal opportunity employer and all qualified applicants will
receive consideration for employment without regard to race, color, religion, sex, sexual
orientation, gender identity, national origin, disability status, protected veteran status, or any
other characteristic protected by law.